Abstract
This is a 24-year-old gentleman with underlying status epilepticus, admitted for septic shock secondary to meningoencephalitis. On pretransfusion investigation, his blood grouping showed ABO discrepancy. His forward grouping showed negative reaction with anti-A, and anti-B while his reverse grouping showed strong 3+ reaction with A1 cell and no reaction with B cell. Repeated tests with new samples also showed similar results. Subsequently, test was repeated at room temperature and 4◦C with prolonged incubation and with enzyme treated cells. Forward grouping showed the same result while the reverse grouping showed enhancement with A1 cell (4+). We suspected B subgroup and proceeded with anti-H lectin test which showed 4+ reaction. Adsorption and elution study confirmed the presence of antigen B on patient’s RBCs. Therefore, it is necessary to resolve all discrepancies to ensure safe transfusion practice.International Journal of Human and Health Sciences Vol. 08 No. 01 Jan’24 Page: 99-102
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