A rapid test for detection of tribenuron-methyl resistance in sunflower

Aleksandra Dimitrijevic,Gordana Surlan-Momirovic,Branislav Kovacevic,Ivana Imerovski,Dragana Miladinovic,Goran Malidza,Sandra Cvejic,Sinisa Jocic

doi:10.5937/ratpov53-8412

Abstract

A rapid laboratory test for detection of tribenuron-methyl resistant sunflower genotypes was developed. Four homozygous and four heterozygous tribenuron-methyl resistant hybrids, as well as a susceptible one were grown on MS medium supplemented with different concentrations of herbicide (2.0 µM, 2.5 µM, 3.0 µM, 3.5 µM and 4.0 µM) and with pH either 7 or 8. The effect of medium pH and herbicide concentration on above-ground part and root mass of sunflower seedlings was observed. The test enabled visual discrimination between resistant and susceptible genotypes in 5 days, and discrimination between homozygous and heterozygous resistant genotypes in 12 days. All tested herbicide concentrations and morphological parameters were found to be suitable for discrimination of tribenuron-methyl susceptible genotypes. The best pH and herbicide concentration combination and morphological parameter for discrimination between homozygous and heterozygous resistant sunflower genotypes were 3.0 µM at pH 7 and root mass, respectively.

Highlights

Herbicide treatment had no effect on 3 homozygous resistant genotypes (HoF, HoP and HoM), it had a negative effect on HoT at 4.0 μM, where a decrease of AGM was observed (Figure 4)
At pH 8, herbicide treatment led to decrease of root mass (RM) in all tested heterozygous resistant genotypes, except for genotype HtP at 2.0 μM
Herbicide treatment had no effect on RM in all homozygous resistant genotypes tested, except in 4 μM treatment where negative effect of the treatment on HoF was observed (Figure 6)

Summary

Introduction

Plant material Four homozygous (HoF, HoT, HoP, HoM), and four heterozygous TBM resistant (HtF, HtT, HtP, HtM) sunflower hybrids, as well as one TBM susceptible hybrid (Os) were used in the experiment (Table 1). Herbicide treatment had no effect on 3 homozygous resistant genotypes (HoF, HoP and HoM), it had a negative effect on HoT at 4.0 μM, where a decrease of AGM was observed (Figure 4).

Objectives

Results

Conclusion