Abstract
We have modified the method originally developed by Schwartz and Cantor [1] for the extraction of intact yeast chomosomal DNA for pulsed field gel electrophoresis. The modified method allows clinical isolates of Candida albicans to be cultured and the DNA extracted in a 24-or 96-well microtire tray. The DNA is of sufficient qualify to enable the isolates to be electrophoretically karyotyped by pulsed field gel electrophoresis, or digested with a restriction endonuclease for subsequent restriction fragment length polymorphism analysis. Several microtire trays can be processed at one time. This makes it possible to prepare DNA from hundreds of clinical isolates, rapidly and with a minimum amount of labor. Further, it would be relatively easy to adapt this method to enable the extraction of DNA from any type of cell, prokaryotic or eukaryotic.
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