Abstract
A method is described for rapid separation of thymocytes from suspensions by centrifugation of the cells through silicone oil. The usefulness of several radioactive compounds as markers for the trapped extracellular water space and the total water space of the cell pellets was investigated. According to the results obtained with [ 3H]inulin and [ 3H]methoxyinulin, the extracellular water space comprised approximately 12% of the total water space as determined by 3H 2O or C 35S(NH 2) 2. The thymocytes appeared to be undamaged after the separation as judged by their oxygen consumption, sodium content, and dexamethasone binding.
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