Abstract
MOTULSKY et al.1 have reported the adaptation of the spectrophotometric glucose-6-phosphate (G6P) dehydrogenase estimation2 to a visual qualitative test; the rate of decolorization of incorporated brilliant cresyl blue, acting as an electron acceptor, enabled red cells with normal and deficient enzyme activity to be distinguished (see Vella3 for description). Some brands of brilliant cresyl blue were found to be far more effective as electron acceptors than others, requiring standardization of the decolorization time for each particular dye to assess normal enzyme activity4.
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