Abstract
A rapid and comprehensive sample preparation method used to extract adriamycin from plasma has been investigated. The samples were passed through an Adsorbex extraction column with an elution solvent. The eluate was directly analyzed by reversed-phase high performance liguid chromatography (HPLC) with fluorescence detector. This solid/liquid extraction method is simpler than the conventional liquid/liquid extraction method. Different elution solvents were used to extract the adriamycin from samples with and without serum. The mobile phase was found to be the optimal elution solvent: 5 mM orthophosphoric acid 62.5% in methanol; acetonitrile; isopropanol = 15:15:7.5, pH 3.2. This method has the merits of better recovery (98%), higher reproducibility, and reguires shorter time and consumes less solvent.
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