A rapid reverse transcription-PCR assay for F + RNA coliphages to trace fecal pollution in Table Rock Lake on the Arkansas–Missouri border

Abstract

Source determination of fecal contamination is imperative to efficiently reduce the fecal material load to environmental waters. This study developed primer pairs targeting three F + RNA bacteriophages and a simple filtration sampling method to enumerate and identify coliphages in environmental waters. Water samples were collected seasonally for one year from the watershed of Table Rock Lake on the Arkansas–Missouri border in areas predisposed to fecal contamination. Collected samples were analyzed quantitatively with most probable number and plaque assays and qualitatively with reverse transcription-PCR. We demonstrated the usefulness of F + RNA coliphages as an indicator of fecal contamination, but were unable to distinguish between human and non-human sources. F + coliphage numbers in Table Rock Lake showed seasonal variation with the highest level of coliphage presence during the January sampling event.