Abstract
A method for the separation of free fatty acids from other lipids is presented. Silicic acid treated with isopropanol-KOH and washed with ethyl ether serves as the column packing. The sample is introduced in ethyl ether, and neutral lipids are eluted with this solvent. Fatty acids are removed from the column with 50 ml of 2% formic acid in ethyl ether followed by 75 to 100 ml of ethyl ether. Phospholipids are retained on the column. The results of 26 analyses of fatty acids mixed with various other lipids showed an average recovery of 98.3% for free fatty acids.
Highlights
This paper describes a rapid, quantitative method for the separation of free fatty acids (FFA) and other lipids
Infrared spectrophotometry showed the glyceride fraction to be free of carboxyl absorption while the FFA fractions were free of absorption due to ester linkages
The data presented in these tables demonstrate the efficiency of the method in separating glycerides and FFA
Summary
A method for the separationof free fatty acidsfrom other lipidsis presented. Silicicacid treated with isopropanol-KOH and washed with ethyl ether serves as the column packing. The results of 26 analyses of fatty acids mixed with various other lipids showed an average recovery of 98.3% for free fatty acids. Anion exchange resins appeared to offer the most promise. Our results with both Amberlite IRA-400@and Dowex 3@. (1) would remove the FFA from a lipid mixture Quantitative recovery of these acids from the column was virtually impossible even under drastic conditions of elution. Gas-liquid chromatographic analyses of the eluted acids showed, that fatty acids fiom previous use were exchanging with the FFA of the sample. This paper describes a rapid, quantitative method for the separation of FFA and other lipids
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