Abstract
5-Hydroxyindoleacetic acid in urine adjusted to 0.1N with HCl, is selectively adsorbed on G-10 Sephadex. Interfering fluorophores are then leached out and the acid eluted with 0.02N NH 4OH, the eluate adjusted to 3N with concentrated HCl and estimated by spectrophotofluorimetry. The method is simple and quick, and its high degree of specificity and sensitivity enables it to be used on urine volumes of less than 1 ml. Recoveries of 90% and more have been obtained with 5-hydroxyindoleacetic acid added to urine.
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