Abstract
A rapid quantitative assay for fibrin-stabilizing factor (factor XIII, FSF), a modification of the method of Lorand and Dickenman in which the ability of the tested material to prevent dissolution of a FSF-free fibrin clot in monochloroacetic acid is determined, is presented. Plasma is added to an FSF-free fibrinogen solution which is clotted with FSF-free thrombin in a tuberculin syringe. The end of the syringe is immersed in monochloroacetic acid at 20 C. The amount of clot dissolved after 20 minutes, as measured by the graduations on the syringe, is inversely proportional to the FSF content of the plasma added. FSF values obtained by this method in a series of normal adults and newborns had 87 and 84% correlation with results obtained by the dansyl cadaverine method of Lorand and co-workers. The mean value for adult bloods was 104%, ±2 S.D. 55-154%, and that for cord bloods was 61%, ±2 S.D. 36-87%.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
