Abstract

A new micromethod for the determination of serum lipase activity is presented. Small quantities of serum are incubated for 30 min at 40° in a substrate consisting of an olive oil suspension in the presence of deoxycholic acid. The pH is 8.5. The hydrolyzed fatty acids are determined with a photometric technique, with a slight modification of the method of Duncombe. The normal values are lower than 20 I.U.

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