A rapid multiclass method for antibiotic residues in goat dairy products by UPLC-quadrupole/electrostatic field orbitrap high-resolution mass spectrometry

Lu Zhang,Qiang He,Liang Shi,Ying Li

doi:10.1186/s40543-021-00268-4

Lu Zhang, Qiang He + Show 2 more

Open Access

https://doi.org/10.1186/s40543-021-00268-4

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Abstract

BackgroundSulfanilamides, quinolones, nitroimidazoles, tetracyclines, cephalosporins, macrolides, and β-lactam are common tools in agriculture and can be found in animal-based foods such as goat milk and goat dried milk. To evaluate the risk of these species, reliable analytical methods are needed for accurate concentration determination, especially in goat milk and goat dried milk.MethodWe describe a method based on PRiME extraction coupled with UPLC-quadrupole/electrostatic field orbitrap high-resolution mass spectrometry to accomplish this task.ResultUnder optimal conditions, the limit of quantification for all antibiotics was 0.5–100 μg/L in goat milk and goat dried milk samples. The recoveries were 60.6–110.0% for goat milk and 60.1–109.6% for goat dried milk with a coefficient of variation less than 15%. The detection limits were 0.5–1.0 μg/kg. The limits of quantification for the analytes were 5.0–10.0 μg/kg. Finally, the method was used to screen veterinary antibiotics in 50 local goat milk and goat dried milk samples; metronidazole and enrofloxacin were detected in goat milk.ConclusionThis method offers good reliability and the capacity for simultaneous detection can be used to detect residual contents and evaluate health risks in goat milk and goat dried milk.

Highlights

Veterinary antibiotics are widely used to prevent infections, increase reproduction, and improve animal husbandry (Han et al 2015; Javorska et al 2017; Li et al 2016; Tran et al 2016; Reinholds et al 2016; SerraCompte et al 2017; Cámara et al 2013)
Under the same determination conditions, DICL and penicillin V (PEV) were weak retention on a Waters ACQUITY UPLC® BEH Shield RP 18 (100 mm × 2.1 mm, 1.7 μm), and CLOX and OXAC were unreserved on a Waters ACQUITY UPLC® HSS T3 column (100 mm × 2.1 mm,1.8 μm)
The response function was linear with a coefficient (r2) of 0.9906–0.9971 for milk samples and 0.9901–0.9998 for dried milk samples (Table 3).The sensitivity was evaluated via the limit of detection (LOD) and limit of quantification (LOQ)

Summary

Introduction

Veterinary antibiotics are widely used to prevent infections, increase reproduction, and improve animal husbandry (Han et al 2015; Javorska et al 2017; Li et al 2016; Tran et al 2016; Reinholds et al 2016; SerraCompte et al 2017; Cámara et al 2013) These drugs are often used in discriminately in cattle and goat feeding (Zorraquino et al 2011), which can lead to adverse human health effects, especially for infants and children who consume large amounts of dairy products (Li et al 2019; Li et al 2017). To evaluate the risk of these species, reliable analytical methods are needed for accurate concentration determination, especially in goat milk and goat dried milk

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