Abstract
A nonsense mutation in the mouse leptin gene causes genetic obesity. As a result of extensive research in the field of obesity, the use of leptinob mice is widespread. This mutation renders mice sterile, creating the need to breed heterozygous mice. For this reason, leptinob genotyping is necessary. To date, gel-based assays have been used for genotyping. Using the Invader Plus assay for single nucleotide polymorphism (SNP) detection, we have developed a gel-free microplate SNP assay for genotyping leptinwt and leptinob alleles.
Highlights
A nonsense mutation in the mouse leptin gene causes genetic obesity
Leptin was discovered by positional cloning of a locus underlying the morbid obesity of the obese mutant mouse [1], a spontaneously occurring mutant that was discovered at the Jackson Laboratory in 1950 [2]
The obese mouse is widely used in studies of obesity and diabetes
Summary
A nonsense mutation in the mouse leptin gene causes genetic obesity. As a result of extensive research in the field of obesity, the use of leptinob mice is widespread. The pioneering studies of Coleman and others [3,4,5] showed that certain mouse strains become diabetic when made obese through the introgression of the leptinob allele These studies have inspired numerous investigators to combine the leptinob allele with other mutations to gain new insights into the molecular, genetic, and physiological processes underlying resistance and susceptibility to obesity-induced type 2 diabetes [6, 7]. This strain is used extensively in nutritional and pharmacological studies aimed at the treatment of obesity and diabetes. Single-stranded conformational polymorphism analysis, involves gel electrophoresis and is quite labor-intensive [10]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
