Abstract
Cellular long-chain fatty acids of 16 yeast strains, representing nine Kluyveromyces species, were extracted from yeast cells by saponification and analyzed as methyl esters by gas-liquid chromatography (GLC). When these species were grown under the same conditions, each produced a distinctive fatty-acid ‘fingerprint’ characterized by certain fatty acid compositions. With this method, it was possible to distinguish on a qualitative basis between the nine species within 4 h after they were obtained from 48 h cultures as compared with 7 to 10 days for more conventional methods. Factors such as speed and sensitivity of this method make it an attractive alternative to conventional laboratory tests for distinguish between yeasts.
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