Abstract
Frequently used methods for the demonstration of cross-striations of muscle fibers are Mallory's phosphotungstic acid hematoxylin (PTAH) (Mallory 1939 and Heidenhain's iron hematoxylin (McManus and Mowry 1960). Both require long staining periods; in addition, Mallory's method specifies fixation or mordanting in Zenker's fluid. A modified Mallory's PTAH procedure has been developed to accelerate the staining of formalin fixed tissue (Bohacek 1966). Preparation of solutions and actual staining time are still lengthy. The method of Menzies (1961) using bromphenol blue, though no mordanting is required, specifies 4-24 hours staining. None of these techniques are practical when time is a major factor. This report describes a staining procedure which makes the differentiation of muscle cross-striations quick and reliable.
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