Abstract

A simple method of studying the abilities of different compounds to inhibit urease activity in soils is described. It involves determination of the effect of the test compound on the amount of urea hydrolyzed by incubation of soils with urea and toluene at 37°C for 5 hr, urea hydrolysis being estimated by colorimetric determination of urea in the extracts obtained by treatment of the incubated samples with 2 M KC1 containing a urease inhibitor (phenylmercuric acetate). The method is rapid and precise, and it is readily adapted for studies of the rate of inactivation of urease inhibitors in soils.

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