Abstract
A rapid and reproducible method for testing the virulence of Cryptonectria parasitica using American chestnut (Castanea dentata) was developed. Bark- and wood-tissue samples excised from American chestnut tree stems were inoculated with virulent and hypovirulent C. parasitica strains. Samples were incubated at 25 C for 4 days, and virulence was assessed as the area of bark and wood tissue having brown, necrotic cells. Hypovirulent strains damaged an area 2.16 cm 2 or less of each tissue sample, while virulent strains consistently damaged a greater area (3.62-3.67 cm 2 ) []
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