Abstract
Abstract Eighteen methods were evaluated to quantify reducing sugars in carrot (Daucus carota L.) roots using drops of juice spotted onto chromatography paper. Reducing sugar detection with dinitrosalicylic acid was rapid (12 to 30 samples per hr) and correlated very well with values obtained by use of high performance liquid chromatography (r = 0.88 overall, ranging from 0.76 to 0.98 in a genetically diverse range of populations). High reducing sugar, low sucrose (Rs/—) roots could be distinguished from low reducing sugar, high sucrose (rs/rs) roots with little error. This method readily complements soluble solids measurement.
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