Year-end Sale % OFF 
Abstract
We have developed a rapid extraction method using low salt concentration buffer for the isolation of low-molecular-weight RNA from Arabidopsis tissues. The method was quick and efficient, and the small scale extraction process took no more than 1 hour, while yield and RNA quality were comparable with those of previously reported. The LMW RNA isolated using this method was high quality, abundant in small RNA and free of high molecular weight RNA. This method can be used to extract low-molecular-weight RNA for the purpose of small RNA cloning and detection, and library construction.
Highlights
We report a novel and efficient method for the extraction of LMW RNA from plant tissues, which is convenient and less time consuming than current methods
We have developed a rapid extraction method using low salt concentration buffer for ly the isolation of RNA from Arabidopsis tissues
The low-molecular-weight (LMW) RNA isos lated using this method was high quality, abundant in small RNA and free of high molecu ular weight RNA. This method can be used to l extract low-molecular-weight RNA for the puria pose of small RNA cloning and detection, and library construction
Summary
We report a novel and efficient method for the extraction of LMW RNA from plant tissues, which is convenient and less time consuming than current methods. We have developed a rapid extraction method using low salt concentration buffer for ly the isolation of RNA from Arabidopsis tissues.
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
