Abstract
AbstractA rapid method for differentiation of Xanthomonas campestris pv. mangiferaeindicae from other Xanthomonads and phylloplane inhabitants is described. Several selective media were evaluated for selective enrichment of X. campestris pv. mangiferaeindicae from mango plants. Boost broth supplemented with cycloheximide, methyl violet and methyl green enhanced growth of epiphytic X. campestris pv. mangiferaeindicae but not other phylloplane organisms. The identity of X. campestris pv. mangiferaeindicae selectively enriched from mango plant material was confirmed with monoclonal antibodies in an Enzyme‐Linked Immunosorbent Assay. Preliminary field evaluations showed that selective enrichment and confirmation by ELISA could successfully detect latent infections of X. campestris pv. mangiferaeindicae in mango plants.
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