Abstract
ABSTRACTThe bitter component, limonin, was quantified in orange and grapefruit juices after preliminary separation on a C‐18 cartridge, or by direct injection of the juice onto a C‐18 or C‐8 high performance liquid chromatographic (HPLC) column. The lower limit of detection for limonin was about 1 ppm on a 2.1 mm i.d. microbore column and 2 ppm on a 4.6 mm i.d. analytical column. In a few orange juice samples, the preliminary separation step was necessary to eliminate interfering substances prior to HPLC separation.
Published Version
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