Abstract
A convenient, rapid colorimetric assay system has been developed in order to determine cell viability of populations of mammalian cells encapsulated using a poly-L-lysine/ alginate encapsulation system. The method is based on the use of 3-(4,5-dimethylthiozol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and involves direct incubation of the capsules with the reagent. This is followed by direct solubilization of the resulting formazan product in dimethyl sulfoxide (DMSO). In order to demonstrate that this assay method may be generally applicable to alginate-based carrier systems, viability of cells immobilized in alginate beads, as distinct from encapsulation in the poly-L-lysine/alginate system, was also determined using the in situ method.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
