Abstract
Sera from adults with toxoplasmosis and individuals with anti nuclear antibodies were examined for the presence of specific IgM and IgG antibodies and for the occurrence of anti-immunoglobulin (‘RF’) activity both before and after absorption with freeze-dried preparations of protein A-containing Staphylococcus aureus. Such absorption removed most of the IgG present in sera without interfering with the detection of IgM antibody by immunofluorescence, but was relatively ineffective in the removal of ‘RF’. ‘RF’ activity, demonstrable by slide agglutination, was removed by absorptino with latex coated with IgG. In order to prevent the possibility of false positive IgM staining in immunofluorescence tests, it is concluded that sequential absorption with latex-IgG particles and protein A-positive staphylococci is essential. Such absorption is technically simple and can be performed on small volumes of serum.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
