Abstract
AbstractTwo methods are described for determining methionine hydroxy analogue free acid (HMB) in supplemented poultry feeds. The procedure is based on extraction of analogue from the feed, followed by high‐pressure liquid chromatographic measurement of the HMB directly after extraction or, more rigorously, after extraction and hydrolysis of oligomers to monomer. Recoveries of HMB over the supplementation range 0.08–0.25% (by wt) are 98% with coefficients of variation of 5.4% in commercial poultry feeds. A 98% correlation coefficient is obtained when the analyses are compared with the gas chromatographic method.
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