Abstract
A green, rapid and sensitive high performance anion-exchange chromatographic (HPAEC) method for the determination of saccharin in environmental water samples is reported. A linear range of 5 μg/L to 28.2 μg/mL was found with a theoretical limit of detection of 5 μg/L based on a signal-to-noise ratio of three. No interferences were seen for sodium cyclamate, acesulfame K, aspartame, nitrite, nitrate and chlorate. Following a simple filtration sample pre-treatment a percentage recovery of 92.9 % and an associated coefficient of variation of 3.8 % was found for a sample fortified with 28.2 ng/mL saccharin. Separation was achieved within 2.5 minutes using a SAP 100 mm × 4.6 mm, 8 μm strong anion exchange analytical column with a mobile phase of 0.1 M NaCl at a flow rate of 1.5 mL/min. The develop method avoids the use of potentially environmentally hazardous reagents and minimises the production of waste.
Highlights
Surface waters, such as rivers, streams, lakes and ponds are important ecosystems providing resources in the form of drinking water, food, irrigation for agriculture and recreation
E. coli is not a single species [5]; certain genera of the coliform group such as Proteus and Aerobacter are normally found outside the human intestinal tract in soil and E. coli can be found in the intestines of all warm-blooded animals [6]
Concentrations of NaCl were investigated between 89 mM and 1.0 M in order to optimise the chromatographic separation of saccharin
Summary
Surface waters, such as rivers, streams, lakes and ponds are important ecosystems providing resources in the form of drinking water, food, irrigation for agriculture and recreation. 1,2-Benzothiazol-3(2H)-one 1,1-dioxide (i) is an artificial sweetener commonly used in wide range products as a sugar substitute in food, beverages, pharmaceuticals, personal care products and animal feed It is excreted from the body essential unchanged [7] and as a result detectable levels have been determined in sewage influents [8,9,10,11,12,13,14,15,16]. Anion exchange techniques require aqueous based mobile phases which represent a green alternative to other chromatographic approaches. As a result, they present little danger to the user or in disposal. This is believed to be the first report on the application of HPAEC for the determination of saccharin in environmental water samples
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