Abstract
Abstract Simple, rapid methods which have been developed for the separation and quantification of sucrose and fructose in biological materials are presented. The method uses thin layer chromatography. The saccharides are stained with thymol-reagent on the chromatograms. The quantitative determination of red spots of saccharides are performed with a Telechrom video densitometer. The density of the spots are directly proportional to the concentration of the saccharides in a certain range. The saccharides content of the sample may be determined with a standard curve on the same chromatograms. The reproducibility of the methods are characterized by a cofficient of variation of about 4–5%
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
