Abstract
A simple, selective, rapid and precise reverse phase high pressure liquid chromatographic method has been developed for the estimation of cinnarizine from pharmaceutical formulation. The method was developed using MICRA-NPS C18(length×OD×ID =33×8.0×6.0 mm, 1.5 μm) column with a mobile phase consisting of acetonitrile, triethylamine buffer (adjusted to pH 4.5 with 10% w/v potassium hydroxide) and tetrahydrofuran in the ratio 30:66:4 respectively, at a flow rate of 0.5 mL/min. Wavelength was fixed at 253 nm. The developed method was validated for linearity, accuracy, precision, limit of detection and limit of quantitation. The proposed method can be used for the routine estimation of cinnarizine in pharmaceutical dosage form.
Highlights
Cinnarizine (CINN), chemically designated as 1-(diphenylmethyl)-4-(3-phenyl-2-propenyl) piperazine (Figure 1), widely used for prophylaxis and treatment of vertigo associated with Meniere’s disease
Literature survey states the availability of various methods for quantification of CINN in pharmaceutical preparations and biological materials like spectrophotometry, polarography, potentiometry, voltammetry, GC, HPTLC and HPLC
In the present investigation a new, simple, accurate, sensitive and precise RP-HPLC method has been developed for the determination of CINN in bulk and marketed formulation for routine analysis
Summary
Cinnarizine (CINN), chemically designated as 1-(diphenylmethyl)-4-(3-phenyl-2-propenyl) piperazine (Figure 1), widely used for prophylaxis and treatment of vertigo associated with Meniere’s disease. Literature survey states the availability of various methods for quantification of CINN in pharmaceutical preparations and biological materials like spectrophotometry, polarography, potentiometry, voltammetry, GC, HPTLC and HPLC. The reported HPLC method, requires the long run due to higher retention time (as high as 22 min), whereas other analysis methods are not suitable for routine analysis[4,5,6,7,8,9]. In the present investigation a new, simple, accurate, sensitive and precise RP-HPLC method has been developed for the determination of CINN in bulk and marketed formulation for routine analysis. CINN was assayed by reverse phase HPLC method. Standard stock solution of a concentration of 200 μg mL-1 of CINN was prepared using mixture of ethanol and acetate buffer (pH 5.5) in 1:1 proportion (solution A). An aliquot (05 μL) was injected and each chromatogram was recorded. Calibration curves were obtained by plotting the response factor versus the concentration
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