Abstract
A means of determining the antibiotic sensitivity of a known pathogen in a mixture of organisms of varying antibiotic sensitivity is described. Such a technique involved the combination of a rapid antibiotic-sensitivity test in which the criterion of sensitivity was the inhibition of microcolony formation on the surface of agar plates containing antibiotic and the fluorescent-antibody reaction used in the rapid identification of bacteria. It was possible to determine the antibiotic sensitivity of enteropathogenic Escherichia coli in artificially mixed cultures within 5 hours by this method.
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