Abstract
A simple and rapid competitive binding method is described for the determination of unconjugated oestradiol in late pregnancy plasma. Macromolecules (oestrogen receptors) present in rabbit uterine cytosol were used as the binding protein. The bound and free fractions were separated by using dextran-charcoal suspension. The reliability of the method has been discussed. Sensitivity will allow determination of low levels of plasma oestradiol. The method can, therefore, be applied routinely in early and late pregnancies to monitor foeto-placental function.
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