Abstract
A rapid and convenient method for the determination of isoniazid in blood serum or plasma has been described. This method is based on the reaction between INH and sodium pentacyanoammineferroate, which results in a yellowish chromogen whose maximum absorption is at 430 mμ. Factors that influence the reaction are discussed in order to attain the maximal sensitivity. In comparison with other methods for INH estimation, the present method is superior, since it is specific and simple. It can be carried out in not more than half an hour with ordinary laboratory equipment.
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