Abstract

Tocopherols and phytosterols are important components of the unsaponifiable fraction in vegetable fatty foods. Their multiple beneficial effects in nutrition are to some extent well established; nevertheless they continue to be an object of intense research. In this work a simple method for simultaneous determination of both phytochemicals by reversed phase HPLC is proposed. The advantage of this method is a considerable reduction in time with simultaneous information on two types of analytes, whose health effects seem to be complementary. The optimized method is applied to a set of olive and nut oils. The results fill gaps in the literature, and highlight the possible capacity of these phytochemicals to discriminate very similar oils.

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