Abstract
A simple, rapid procedure for the assay of dipeptidyl aminopeptidase III in human erythrocytes has been developed. The cells are lysed with Triton X-100, centrifuged, and the supernatant fractionated on small columns of carboxymethyl Bio-Gel A at pH 6.5. The enzyme is eluted in the void volume and can be obtained completely separated from hemoglobin; it is then assayed by hydrolysis of Arg-Arg-β-naphthylamide. The procedure is suitable for the routine screening of large numbers of human blood samples for their DAP III activity.
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