Abstract
An assay procedure for dopa decarboxylase has been described. The method uses dopa-C 14 as substrate and a butanol extraction under neutral conditions of pH to separate the formed dopamine from dopa. This methods allows a very rapid and particularly simple determination of dopa decarboxylase activity in samples containing low amount of enzyme, such as adrenergic tissues. Dopa decarboxylase and dopamine-β-hydroxylase can be separated and partially purified on the basis of their different sedimentation through a sucrose gradient.
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