Abstract
An accurate and convenient colorimetric method for the determination of serum and urinary lipase is described. The substrate used is α-naphthyl palmitate. The α-naphthol released is coupled in alkaline conditions with the stabilized diazonium salt, Fast Violet B. Pancreatic lipase is activated by sodium cholate, and Teepol 610. Pseudocholinesterase is inhibited by eserine salicylate. The enzyme obeys zero order kinetics to at least 50 I.U., the normal range being 0.5–2 I.U. By using n-propanol the substrate emulsion is destroyed leaving a clear solution for colorimetry. The final colour is stable for 24 h. All reagents are stable for at least one month, with the exception of the substrate emulsion which must be prepared on the day of use. This is, however, an extremely simple operation. The method is rapid, sensitive, uses small quantities of serum and is particularly suitable for batch analysis.
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