Abstract
A rapid and specific high-performance liquid chromatography assay of lamotrigine in human plasma is described. Lamotrigine is extracted with dichloromethane from buffered plasma to which an internal standard has been added. The solvent is directly injected into a 250 x 4.6-mm Spherisorb Silica column and the drug is eluted by using a mixture of methanol, n-heptane, dichloromethane, and 28-30% ammonium hydroxide (20:40:40:0.3 vol/vol) at a flow rate of 1 ml/min. The eluates are detected at 240 nm. The assay requires 250 microliters of sample, and concentrations as low as 0.4 microgram/ml can be measured accurately. The method is linear in the range of 0.4-16 micrograms/ml, with a mean coefficient of correlation (r) > or = 0.997. Within- and between-day relative standard deviations at three different concentration levels (1, 4, and 8 micrograms/ml) are < or = 8.6%.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
