A rapid and simple extraction of anti-depressant drugs by effervescent salt-assisted dispersive magnetic micro solid-phase extraction method using new adsorbent Fe3O4@SiO2@N3

Abstract

In this work, a rapid and simple method is applied using a newly synthesized nanoadsorbent for the extraction and preconcentration of the drugs Amitriptyline (AMT) and Nortriptyline (NRT), which are then determined using high performance liquid chromatography. We focus on the facile synthesis of Fe3O4@SiO2@N3, as a new and effective adsorbent, and the effervescent salt-assisted dispersive magnetic micro solid-phase extraction method. The applied method and modification of the surface of Fe3O4@SiO2 with the nitrogen-rich group lead to an increase in the interaction between the nanoadsorbent used and the analytes, and increase the extraction recovery. The accuracy of the synthesized nanoadsorbent is confirmed by the FT-IR, FE-SEM, XRD, and VSM analytical techniques. In order to obtain the best experimental conditions, optimization of the main variables involved is carried out using the central composite design method. Under the optimum experimental conditions, the limits of detection (LODs), linear dynamic ranges (LDRs), and relative standard deviations (RSDs for n = 5) for NRT and AMT were found to be as follow: LODs, 0.03 and 0.05 ng/mL; RSDs, 2.04 and 1.1 for NRT and AMT, respectively; and LDRs, 0.07–2000 ng/mL. Furthermore, the results obtained show that the nanoadsorbent can be used for five times with an extraction recovery more than 80% and 70% for AMT and NRT, respectively.