A Rapid and Sensitive Method for the Simultaneous Determination of Multipolar Compounds in Caulis Dendrobii by Supercritical Fluid Chromatography Coupled to Ion Mobility Quadrupole Time-of-Flight Mass Spectrometry

Abstract

In this study, matrix solid phase dispersion (MSPD) microextraction combined with supercritical fluid chromatography-ion mobility quadrupole time-of-flight mass spectrometry (SFC/IM-QTOF-MS) was used to analyze the multipolar compounds in caulis dendrobii. The parameters of stationary phase, mobile phase, make-up solution, temperature and back pressure were optimized. The target analytes were gradient eluted in 8 min by supercritical CO2 on Zorbax RX-SIL column. Collisional Cross Section (CCS) values for single and multiple fields were measured. A series of validation studies were carried out under the optimal conditions, and the linear relationship and reproducibility were good. The limits of detection were 1.415~8.824 ng/mL, and the limits of quantification were 4.717~29.412 ng/mL. The recoveries of most compounds ranged from 86.51% to 107.2%. As a consequence, the proposed method was used for the separation and quantitative analysis of active ingredients in caulis dendrobii.