Abstract
A method was developed for the analysis of neutral sugars commonly found in mammalian tissues. It was based on the reaction of reducing sugars with dansyl hydrazine and their separation by reverse phase high-performance liquid chromatography. Sample preparation by this method required only 15 min. Fluorescent detection provided a sensitivity of 10 pmol per injection with an excitation wavelength of 240 nm and an emission longpass wavelength of 550 nm. Alternately, absorbance at 254 nm can be used with a 100-fold drop in sensitivity. The effectiveness of this procedure for the determination of d-glucuronic acid is also discussed.
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