Abstract
An assay for simultaneous quantitation of deoxyribonucleoside triphosphates (dNTPs) in cell extracts is described. Following destruction of ribonucleotides by treatment with periodate and methylamine, dNTPs are separated by high pressure liquid chromatography and quantitated by their ultraviolet absorbance upon elution. Destruction of ribonucleotides is complete (>99.9%), the resulting mixture is suitable for direct injection without further manipulation, and recovery of dNTPs is reproducible and near quantitative. This technique offers a simple and rapid alternative to either DNA polymerase or thin-layer chromatography assays for dNTPs in cellular extracts.
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