Abstract
Electrophoretic separation of seven acidic glycosaminoglycans was accomplished by two-dimensional electrophoresis on a cellulose acetate strip with 0.1 M pyridine/0.47 M formic acid buffer of pH 3 in the first dimension and 0.1 M barium acetate solution of pH 8 in the second. As little as 0.5 μg of acidic glycosaminoglycans can be detected on the cellulose acetate strip after electrophoretic separation. High resolving power and sensitivity of this method make it possible to identify AGG in tissues in as small amounts as biopsy specimens. The separations of chondroitin 4-sulfate from chondroitin 6-sulfate and chondroitin from hyaluronic acid were demonstrated by electrophoresis with 0.1 M cupric acetate solution, pH 5.3. The methods were applied to the separation and identification of acidic glycosaminoglycans in human skin.
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