Abstract
Isolation of high molecular weight DNA is the prerequisite for molecular systematic studies. The tradi- tional lengthy plant DNA isolation protocol by using CTAB detergent has been modified to a simple, rapid and efficient protocol so as to isolate large number of plant samples within a short period of time. This modified pro- tocol has been tested successfully on Chenopodium album, Calotropis procera and Ziziphus mauritiana that signifi- cantly minimizes the time and use of laboratory materials and provides a high yield of DNA in the range of 142.5 lg (Calo1) and 49.5 lg (Zizy2) from 60 mg of silica dried leaves. The yields and quality of genomic DNA are con- siderably affected when the common protocol for DNA isolation is applied to the Chenopodium, Calotropis and
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