A rapid analysis of Bisphenol A using MISPE coupled with HPLC-FLD in tissues of food-producing animals

Vesna Cerkvenik-Flajs,Sabina Šturm

doi:10.1016/j.mex.2021.101351

Abstract

Bisphenol A (BPA) is a highly-produced organic compound of anthropogenic origin with a wide-range use and is ubiquitously present in both living organisms and the environment. A previously published analytical method for testing of the free (aglycone) BPA in foodstuffs was simplified and optimized for sheep muscle tissue, kidney and liver, by using only a single MISPE purification step allied with HPLC-FLD analysis. The recovery rates and RSD values over a concentration range of 1–10 µg/kg were in the range of 67‒86% and 3‒34%, respectively, while linearity in the matrix, represented by the r2, was ≥0.999. LOD values were 0.5‒1 µg/kg and LOQ values were 1 µg/kg. The analytical method used is a contribution to the field of veterinary toxicology and food testing and also proved to be applicable for other food-producing animal species, e.g. pigs, poultry and freshwater fish. The MISPE sorbent material for testing of BPA was reusable, with up to five re-use cycles without significant loss in performance characteristics.•The paper reports on a rapid determination of free (aglycone) BPA in tissues of food-producing animals by HPLC-FLD and presents a substantial improvement of analytical performance with regard to time- and cost-savings, as well as environmental protection.•MISPE is an advanced analytical technology, and the results proved that in a single and simplified SPE step it had good performance characteristics such as selectivity, recovery and precision, allied with low LOD and LOQ values.•It was proved that MISPE BPA cartridges could be used at least five times without significant loss of methodological recovery and precision.

Highlights

molecularly imprinted solidphase extraction (MISPE) is an advanced analytical technology, and the results proved that in a single and simplified Solid-phase extraction (SPE) step it had good performance characteristics such as selectivity, recovery and precision, allied with low limit of detection (LOD) and limit of quantification (LOQ)
On the basis of the reported reusability of the molecularly imprinted polymers (MIPs) by Gallego-Gallegos et al [11] and a recent publication on the successful multiple use of MIP cartridges for determination of ochratoxin A in pig muscle, kidney and liver by Luci [20], we evaluated the possible multiple use of MISPE Bisphenol A (BPA) cartridges for testing the sheep matrices investigated in the current work
The importance of appropriate quality control of sample testing was considered, as blank controls were included with each batch of the fortified samples analysed to avoid and minimize possible artifacts or contamination and ensure appropriate performance characteristics of the BPA analytical method [22]