Abstract
A method for the determination of S-adenosylmethionine O-diphenol O-methyl-transf erase (E.C. 2.1.1.6) is described, basedon the measurement of radioactive metanephrine produced by the action of the enzyme on labelled epinephrine. The metanephrine is extracted by toluene-isoamyl alcohol and estimated in a liquid scintillation counter. Using this method, the activity of the red cells of normal, apparently healthy persons was found to be within the range 20 to 58 × 10 −4 Units per ml of whole blood (mean ± 2 S.D.). No activity was found in the plasma of these subjects. In females taking high oestrogen content oral contraceptives, elevation of catechol- O-methyl-transferase values in the red cells was noted.
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