Abstract
A radioimmunoassay for the measurement of turkey LH (TLH) is described. The antiserum used was developed against purified TLH (No. 70355). Another TLH (CG-C) preparation was used for iodination and as a reference standard. Linear logit vs log dose plots were obtained with standard between 0.10 and 16.0 ng TLH/assay tube. Sera from laying famales, nonlaying females, intact males, castrate males, thyroidectomized males and broody females inhibit bindings of 125I-TLH, yielding dose-response curves parallel to the standard curve. Sera from a hypophysectomized turkey, at levels up to 200 μl/assay tube did not inhibit binding of 125I-TLH. Assay of TLH, added to hypophysectomized sera, resulted in essentially 100% recovery. Luteinzing hormone releasing hormone elicited an increase in immuroreactive LH within 10 min after injection. Castration of male turkeys resulted in elevated immunoreactive LH. Manipulation of the pituitary-thyroid axis yielded conflicting results. Thyroidectomy, triiodothyronine (T 3) and thyroxine (T 4) treatment, and thiouracil feeding had no effect on serum LH, but TRH injection elevated the immunoreactive material. Evidence is presented that suggests TRH releases LH in Aves.
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