Abstract
After extraction, unconjugated estrone, estradiol, and estriol were separated with celite chromatography, with methanol-water as stationary phase, and measured by radioimmunoassay (RIA) using an anti-E1, 17β-hemisuccinate antibody. Separation of bound from free hormone was achieved by dextran-coated charcoal. The precision of the method is approximately 10 per cent for E1 and E3 and 15 per cent for E2. Ten women were followed throughout pregnancy. During the first trimester, plasma values of E1, E2, and E3 were low; then there was a regular increase of plasma E1 and E2 to week 39; estriol showed a biphasic increase. This observation is confirmed by the changes in theE2E3ratio, which tended to decrease from week 20 to 40 of gestation.
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