Abstract
A procedure for the detection of immunoreactive insulin in human plasma is described. The procedure is a rapid radioimmunoassay (3 hr) which uses zirconyl phosphate gel (Z-gel) at a pH of 6.25 to adsorb antibody-bound insulin while leaving free insulin in solution. Thirty-two samples of human plasma which had been assayed for immunoreactive insulin using a commercially available assay (Pharmacia Fine Chemicals, Piscataway, NJ) were also assayed on a blind basis for immunoreactive insulin using the Z-gel technique. A statistical comparison of the data obtained by the two methods yielded a highly significant ( P < 0.01) coefficient of correlation (0.998). A known amount of bovine insulin was added to each of these plasma samples, and the Z-gel assay repeated. The mean percentage recovery of added insulin was 100.4 ± 17.0 (SD). In addition, the insulin response of two healthy individuals to the ingestion of 100 g of glucose was determined using the Z-gel assay. The insulin response curves were similar to those described by other investigators.
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