Abstract
AbstractA sensitive and selective radioimmunoassay has been developed using a colchicine binding monoclonal antibody. The assay procedure uses a charcoal suspension to separate antibody bound and free colchicine and can be performed in less than two hours. Using the high affinity antibody, as little as 0.3 ng/ml of colchicine in serum can be detected. These results provide the framework for a fully validated clinical assay for therapeutic monitoring of colchicine.
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