Abstract
Both inter- and intra-specific maps have been developed in eggplant (Solanum melongena L.). The former benefit from an enhanced frequency of marker polymorphism, but their relevance to marker-assisted crop breeding is limited. Combining the restriction-site associated DNA strategy with high throughput sequencing has facilitated the discovery of a large number of functional single nucleotide polymorphism (SNP) markers discriminating between the two eggplant mapping population parental lines ‘305E40’ and ‘67/3’. A set of 347 de novo SNPs, together with 84 anchoring markers, were applied to the F2 mapping population bred from the cross ‘305E40’ x ‘67/3’ to construct a linkage map. In all, 415 of the 431 markers were assembled into twelve major and one minor linkage group, spanning 1,390 cM, and the inclusion of established markers allowed each linkage group to be assigned to one of the 12 eggplant chromosomes. The map was then used to discover the genetic basis of seven traits associated with anthocyanin content. Each of the traits proved to be controlled by between one and six quantitative trait loci (QTL), of which at least one was a major QTL. Exploitation of syntenic relationships between the eggplant and tomato genomes facilitated the identification of potential candidate genes for the eggplant QTLs related to anthocyanin accumulation. The intra-specific linkage map should have utility for elucidating the genetic basis of other phenotypic traits in eggplant.
Highlights
The eggplant (Solanum melongena L.) is the third most important solanaceous crop, with a global production level in 2010 of,41.8Mt
The remaining 16 loci were associated as triplets or were unlinked; five of the microsatellites and two of the high resolution melting (HRM) markers were assigned to an linkage group (LG) but not ordered in the map
The overall length of the map was 1,390 cM, with individual chromosomes ranging in length between 80.2 cM (E07) and 136.5 cM (E03); the number of loci per chromosome was highest in E02 (66) and lowest in E11 (22) (Table 1)
Summary
The eggplant (Solanum melongena L.) is the third most important solanaceous crop (after potato and tomato, see http://faostat.fao. org), with a global production level in 2010 of ,41.8Mt. The eggplant (Solanum melongena L.) is the third most important solanaceous crop Org), with a global production level in 2010 of ,41.8Mt. The eggplant (Solanum melongena L.) is the third most important solanaceous crop The most recently published intraspecific map (1,285 cM) was developed from two F2 populations using a mixture of microsatellites and gene-based markers, mostly derived from putative orthologs among eggplant, tomato and potato [8]. The latter map has been exploited to map quantitative trait loci (QTL) associated with parthenocarpy [9]
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