A Rabbit Model for Metabolic Studies after Burn Injury

Abstract

Background.A rabbit burn model was developed and characterized, which will allow conduct of repeated, noninvasive and more sophisticatedin vivometabolic studies to explore the pathophysiology of burn injury, owing to its larger blood volume and tissue mass than the rat.Materials and methods.A 20% body surface, full thickness burn was applied to the backs of six anesthetized rabbits by immersion into a boiling-water bath for 10 s, followed by resuscitation with saline. Resting energy expenditure (REE) was measured daily in pre- and postburn periods. Whole body protein kinetics were evaluated using L-[1-13C]leucine tracer, on the preburn and the third postburn day. Fasting plasma glucose was also measured.Results.A significant elevation of REE began on the second postburn day and reached 34 ± 8% above the preburn level (P< 0.05, pairedttest) on the third postburn day. The fasting plasma leucine flux and oxidation were significantly elevated from their preburn levels (bothP< 0.05), indicating an accelerated proteolysis and a more negative body protein balance (P< 0.05); however, the rate of whole body protein synthesis did not differ significantly pre- and postburn injury. Fasting plasma glucose also increased (P< 0.001) on the third postburn day. The burn scar remained intact during the study, without any sign of infection.Conclusions.The metabolic changes observed in this animal model can be attributed to burn injuryper seand they mimic those for flow phase in burn patients. This rabbit burn model should be suitable for exploring mechanistic aspects of the burn-induced changes in metabolism and nutrient balance.