Abstract
The relationship of various parameters to the biosynthesis of connective tissue proteins and polysaccharides in rabbit sponge capsular tissue in vitro is presented. A linear relationship of isotope incorporation to microsomal RNA and DNA content per flask was found. Enzymatic analysis of 1 M NaCl-soluble products indicates that the [ 14C]proline-rich macromolecule is at least 50% procollagen and d-[I- 14C]glucosamine-containing polymer is 60–70% hyaluronate and 28% glycoprotein. [ 14C]Tyrosine was found to be rapidly incorporated into 1 M NaCl-soluble, collagenase-resistant proteins. Applications of these methods to the study of the connective tissue requirements for glucose and other simple metabolites is presented. The effects of alcohol, steroid hormones and insulin on connective tissue synthesis is also included.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
